In the process of cell fractionation, cell lysis is the first step! It is also used in the processes of isolating cell organelles, purification and especially in protein extraction! In simple words, cell lysis is the solution to many research methods in proteomics. Over the years, several lysis techniques have been developed so that the results obtained are of the highest quality.
The reason behind it all was simple, to begin with. It is to get pure samples from all the known species of living organisms! When it comes to genetics, it is critical for the sample to be pure and intact in terms of its properties, target molecule and integrity of the subcellular structure.
Tissue or bodily fluid – which is better for preparing protein samples!?
When one is looking at the ideal processor rather protocol of preparing a protein sample, there is none, to begin with! Protocols for sample preparation must be altered as per the demands of the situation. Several factors come into play in the time of preparing a protein sample such as:
Keeping all the above factors in mind, it is safe to say that bodily fluids like plasma or urine are ideal when it comes to extracting a sample. It is due to the fact that bodily fluids are protein solutions with less homogenous properties. In order to get your hands on the target protein molecule, all you need to do is to conduct a series of controlled yet minor manipulative techniques!
Obtaining samples from tissue specimens is a whole different arena, to begin with! It involves major and painstakingly complex manipulative techniques. Only after that, one can break down complex tissue architecture to control enzymatic activities so that the target protein molecules can be obtained!
So to sum it up, it is better to go for bodily fluids when one is planning to extract protein samples from a specimen.
An overview of contemporary and traditional cell lysis methods
If one takes a closer look at history, physical lysis was the preferred method when one was looking to disrupt cells and extract cellular content. The method soon fell out of favor as it involved strict protocols that are complex to follow and results were often undesirable since a little difference in apparatus can lead to unwanted results! On top of that, physical cell disruption methods are less efficient when compared to contemporary laboratory techniques! The most popular cell lysis method is reagent based cell lysis which provides better results when one compares the same with physical disruption methods.
The goal of cell lysis is to test the overall functions of a particular protein molecule. To make sure that the results are satisfactory, one needs to make sure that they are choosing the right lysis reagent if they want to extract the protein molecule of interest! For best results, it is best to get your samples from professionals like BioChain. BioChain uses patented Total Protein Extraction Kit. It allows them to give samples that are known to deliver satisfactory results. For more details feel free to visit https://www.biochain.com/products/protein/total-protein-lysate